Primer
Part:BBa_K4496014:Design
Designed by: Larissa de Souza Kawanisi, Awana da Silva Lima, Paula Macedo Cunha, Gabriel Kimijima Itaziki Group: iGEM22_USP-EEL-Brazil (2022-09-20)
Primer (FWD) optimized for BJ46a gene amplification in Escherichia coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 19
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This primer was designed from the BJ46a circuit sequence optimized for Escherichia coli. For this, the first 8 amino acids were chosen and tested for hairpin.
Source
https://parts.igem.org/Part:BBa_K4496003